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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Exp Mol Pathol. 2012 Apr 16;93(1):40–49. doi: 10.1016/j.yexmp.2012.04.002

Fig. 6.

Fig. 6

Effect of rapamycin treatment on oval cell abundance and proliferation in rats treated with 2-AAF followed by 2/3 partial hepatectomy (PHx). (A) Western immunoblotting for phospho-S6Ser235/236 and total S6 was performed on total liver homogenates from vehicle (V) or rapamycin (Rapa) treated rats placed on the 2-AAF PHx protocol and harvested 7 days post-hepatectomy. (B) Cryosections from these animals underwent immunofluorescent staining for an oval cell marker BD.2 and a marker of DNA synthesis EdU or (C) a hepatocyte marker H.4 and EdU. Slides were counterstained with DAPI. Images were acquired at 40×. Scale bar: 50 μm. Image analysis was undertaken as described in the Supplemental Information.