Figure 1. Renal morphology and immunohistochemical staining for type IV collagen in the experimental groups.
A. Representative light microscopic appearance of glomeruli (PAS and PAM staining) and immunohistochemistry of Col4 for control rats (a, e, and i), STZ-induced diabetic SD rats, (b, f, and j), Zucker lean rats(c,g, and k), and Zucker fatty rats (d, h, and l). Bar = 100 μm.
B. Morphometric analysis of PAM positive staining area. The glomerular PAM positive area was measured as described in materials and methods. n=6 in each group, *P<0.05. C. Morphometric analysis of the glomerular expression of Col4. The glomerular staining area of Col4 was measured as described in materials and methods. n=6 in each group, *P<0.05. The data are expressed as means ± SD. Cont.= control rats, DM=STZ-induced diabetic SD rats, ZL=Zucker lean rats, ZF=Zucker fatty rats, ns=not significant.