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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Kidney Int. 2011 Jan 12;79(8):883–896. doi: 10.1038/ki.2010.526

Figure 5. NF-κB activation and effect of RBX, PKCβ inhibitor on p-Akt, p-eNOS and p-GSK3α in the glomeruli of diabetic SD rats and ZF rats.

Figure 5

A. Representative immunoblots of NF-κB (p65) from nuclear proteins of glomerular and tubular fractions. Data from three experiments were quantitated by densitometry. n=6 in each group, **P<0.001.

B. Transcriptional binding activity assay of NF-κB in glomerular and tubular fractions. n=6 in each group, **P<0.001.

C and D. Representative immunoblots of p-Akt from glomerular fractions. Data from three experiments were quantitated by densitometry. A) Cont. vs. Cont.+RBX vs. DM vs. DM+RBX, n=6 in Cont., Cont.+RBX and DM. n=5 in DM+RBX, **P<0.001 vs. Cont./insulin(-)/RBX(-). ##P<0.001 vs. Cont./insulin(+)/RBX(-). ††P<0.001 vs. DM/insulin(+)/RBX(-). B) ZL vs. ZL+RBX vs. ZF vs. ZF+RBX n=6 in each group, **P<0.001 vs. ZL/insulin(-)/RBX(-). ##P<0.001 vs. ZL/insulin(+)/RBX(-). †P<0.05 vs. ZF/insulin(+)/RBX(-).

E and F. Representative immunoblots of p-eNOS from glomerular fractions. Data from three experiments were quantitated by densitometry. C) Cont. vs. Cont.+RBX vs. DM vs. DM+RBX, n=6 in Cont., Cont.+RBX and DM. n=5 in DM+RBX, **P<0.001 vs. Cont./insulin(-)/RBX(-). ##P<0.001 vs. Cont./insulin(+)/RBX(-). ††P<0.001 vs. DM/insulin(+)/RBX(-). D) ZL vs. ZL+RBX vs. ZF vs. ZF+RBX n=6 in each group, **P<0.001 vs. ZL/insulin(-)/RBX(-). ##P<0.001 vs. ZL/insulin(+)/RBX(-). ††P<0.001 vs. ZF/insulin(+)/RBX(-).

G and H. Representative immunoblots of p-GSK3α from glomerular fractions. Data from three experiments were quantitated by densitometry. E) Cont. vs. Cont.+RBX vs. DM vs. DM+RBX, n=6 in Cont., Cont.+RBX and DM. n=5 in DM+RBX, **P<0.001 vs. Cont./insulin(-)/RBX(-). ##P<0.001 vs. Cont./insulin(+)/RBX(-). ††P<0.001 vs. DM/insulin(+)/RBX(-). F) ZL vs. ZL+RBX vs. ZF vs. ZF+RBX n=6 in each group, **P<0.001 vs. ZL/insulin(-)/RBX(-). ##P<0.001 vs. ZL/insulin(+)/RBX(-). ††P<0.001 vs. ZF/insulin(+)/RBX(-). These data are expressed as means ± SD. Cont.=control rats, Cont.+RBX=control rats treated with ruboxistaurin, DM=STZ-induced diabetic rats, DM+RBX=STZ-induced diabetic rats treated with ruboxistaurin, ZL=Zucker lean rats, ZL+RBX= Zucker lean rats treated with ruboxistaurin, ZF=Zucker fatty rats, ZF+RBX= Zucker fatty rats treated with ruboxistaurin, G=glomeruli, T=tubules, ns=not significant.

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