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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Glycobiology. 2003 Feb 20;13(6):497–507. doi: 10.1093/glycob/cwg051

Fig. 7.

Fig. 7

HPAEC-PAD analysis of N-glycans from GST-SfManI. GST-SfManI was affinity-purified from AcGST-SfManI-infected SfSWT-1 (A–B) or SfSWT-3 (C–E) cells cultured in medium containing serum (A–B), serum-free medium (C), or serum-free medium supplemented with 10 mM N-acetylmannosamine (D–E), as described in Materials and methods. Each protein was then digested with PNGase-F alone (A, C, D) or with PNGase-F and neuraminidase (B, E), and the N-glycans were recovered and analyzed by HPAEC-PAD, as described in Materials and methods. The elution positions of standards, including a digalactosylated biantennary N-glycan (1), a monosialylated N-glycan (2), free Neu5Ac (3), and a disialylated biantennary N-glycan (4), are indicated by the arrows.