Figure 6.

Interaction between p53 and NF-κB p65 governs the activated secretion profile in ovarian fibroblasts. (A) Co-IP from precleared lysates of the same cell lines listed above using either p53-linked or p65-linked agarose beads, followed by Western immunoblot (IB) analysis for p65, phospho-p65 at serine 276, or p53 (N = 2). (B) Normal ovarian fibroblasts expressing hTERT, p65 attenuation, p53 attenuation, or p53 and p65 attenuation viral constructs were transiently co-transfected with dual-luciferase reporter plasmids bearing either wild-type (wt) or mutated (mut) NF-κB promoter driving firefly luciferase [48] and control plasmid bearing CMV intermediate promoter controlling Renilla luciferase (pRL-CMV). The ratio of detection of firefly/Renilla luciferases in relative light units was standardized according to total protein concentration for each lysate (N = 4).