A and B. Priming of PBMCs with IFN-γ and IL-15 prior to stimulation with the TLR7/8 agonist 007 significantly increases IL-12 p70 production (A) but only priming with IFN-γ significantly increases levels of IFN-α (B).
PBMC from Sezary syndrome patients were cultured with media, IFN-γ, or IL-15 for 4h followed by 20h stimulation with 007, a TLR7/8 agonist. Cytokine levels were measured in cell-free supernatants by ELISA: A. IL-12p70; B. IFN-α. Data represent mean ± standard deviation of tested individuals (n=10). ** p<0.005; * p<0.05