Table 1.

Tissue culture-based candidate biomarkers discovery in different cancers.

Cancer types	Cell lines used	Biomarker identified	Clinical relevance	Quantitative techniques used	Reference
Breast cancer	21T series of Breast cancer cell lines  HMT-3522-S1, MFM223, HCC202 and HCC2218, HCC1599, HCC1143, HCC1937, MCF7, MCF10A, MDA-MB-453	TIMM 17A  IDH2, CRABP2, SEC14L2	Disease prognosis  Disease progression and monitoring	SILAC and LC-MS/MS  SILAC and MALDI-MS/MS	[5]  [44]
Prostate cancer	PC3, LnCAP, 22Rv1	Follistatin, chemokine (C-X-C motif) ligand 16, Pentraxin 3, and spondin 2	Disease progression and monitoring	Two-dimensional chromatography and tandem mass spectrometry  2D-DIGE, MALDI-MS/MS	[76]  [92]
Lung cancer	1198 and 1170-I, BEAS-2B and 1799  CL1-0 and CL1-5	PGP9.5, TCTP, TIMP-2, and TPI  KPNA2	Disease monitoring  Disease detection and progression monitoring	2DE and MS  SILAC, LC-MS/MS	[81]  [46]
Gastric cancer	AGS and MKN7	GRN	Disease detection and monitoring	2D-LC-MS/MS and iTRAQ	[93]
Pancreatic cancer	PANC1, BxPc3, MIA-PaCa2, SU.86.86	Anterior gradient homolog 2, syncollin, olfactomedin-4, polymeric immunoglobulin receptor, and collagen alpha-1(VI) chain	Early disease detection and monitoring	LC-MS/MS, ELISA	[94]
Colorectal cancer	Tumor samples were cultured in vitro	EFEMP2	Detection and monitoring	1D-LC-MS/MS	[95]
Head and Neck cancer	SCC4, HSC2, SCC38, and AMOSIII	alpha-enolase, peptidyl prolyl isomerase A/cyclophilin A, 14-3-3 z, heterogeneous ribonucleoprotein K, and 14-3-3 s	Disease detection and progression monitoring	LC-MS/MS, western blot	[96]
Oral cancer	OEC-M1 and SCC4  OEC-M1 and SCC4	Mac-2 BP  Guanylate-binding protein 1 (GBP1)	Early detection of disease  Disease detection and progression	MALDI-TOF MS  1D and LC-MS/MS	[97]  [98]
Renal cell carcinoma	786-O, Caki-1, A498, ACHN OS-RC-2, HK-2, HUVEC	FoxM1	Detection and potential drug target	IHC, western blot, ELISA	[99]