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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Biomol Screen. 2009 Sep 2;14(8):956–969. doi: 10.1177/1087057109343207

Figure 3. Confocal microscopy imaging of caspase activation using the DNV fluorogenic substrate.

Figure 3

Hoechst, DNV, Alexa Fluor 633 phalloidin triple staining of HeLa-Empty cells treated with 10 μM Doxorubicin for 72h and imaged on the INCA3000. A. Blue channel: Hoechst staining of nuclei. B. Green channel: NucView488™ staining. C. Overlay of blue and green channel image. D. Overlay of red channel: Alexa Fluor 633 phalloidin staining of actin, with blue and green channels.