Figure 6. Assessment of the stability of the DNV fluorogenic substrate signal.
A. HeLa-Empty cells or B. HeLa-Bcl-XL cells were treated with DMSO control or concentrations of Etoposide (Etop.) ranging from 0.005 μM to 10 μM for up to 72h and imaged every 24h. DNV addition was performed right after preparation of the DNV solution or after 3, 6, 12 or 24h storage on our automated platform in the conditions of screening.