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Effect of treatment on leukocyte subset changes. C57BL/6J mice (n=6 per group) bearing MC38 flank tumors received i.t. injections of DPBS, chitosan alone, IL-12(1μg) alone or chitosan/IL-12(1μg) on days 7 and 14. On day 17, A, tumors, and B, TDLNs were harvested and viable leukocytes stained with fluorescence-labeled antibodies for subsequent flow cytometry analysis. Data are presented as mean ± standard deviation. *P<0.05 vs. DPBS; ** P<0.05 vs. chitosan; *** P<0.05 vs. IL-12.
