A. hydrophila SSU-R strains |
Rifampin resistance strain, Rifr
|
Laboratory |
Parental |
pBAD plasmid, Rifr Apr
|
Erova et al. (2006b) |
Dam-overproducing |
pBAD-dam plasmid, Rifr Apr
|
Erova et al. (2006b) |
E. coli K12 strains |
|
|
TOP10 |
F−
mcrA Δ mrr-hsdRMS-mcrBC Φ80lacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG |
Invitrogen |
GM28 |
F−
sup-85 (Am) |
Marinus and Morris(1973) |
GM33 |
F−
dam-3 sup-85 (Am) |
Marinus and Morris(1973) |
GM28 (pGlow-UpgidA) |
GM28 with pGlow-UpgidA plasmid |
This study |
GM33 (pGlow-UpgidA) |
GM33 with pGlow-UpgidA plasmid |
This study |
GM28 (pGlow-Upact) |
GM28 with pGlow-Upact plasmid |
This study |
GM33 (pGlow-Upact) |
GM33 with pGlow-Upact plasmid |
This study |
GM28 (pGlow-TOPO) |
GM28 with pGlow-TOPO vector |
This study |
GM33 (pGlow-TOPO) |
GM33 with pGlow-TOPO vector |
This study |
GM28 gidA mutant |
In-frame gidA mutant of GM28 |
This study |
GM33 gidA mutant |
In-frame gidA mutant of GM33 |
This study |
GM28 gidA mutant (pGlow-Upact) |
GM28 gidA mutant carrying pGlow-Upact plasmid |
This study |
GM33 gidA mutant (pGlow-Upact) |
GM33 gidA mutant carrying pGlow-Upact plasmid |
This study |
GM28 gidA mutant (pGlow-UpgidA) |
GM28 gidA mutant carrying pGlow-UpgidA plasmid |
This study |
GM33 gidA mutant (pGlow-UpgidA) |
GM33 gidA mutant carrying pGlow-UpgidA plasmid |
This study |
Plasmids |
|
|
pGlow-TOPO |
Promoterless vector, Apr Nmr
|
Invitrogen |
pGlow-UpgidA
|
pGlow carrying upstream region of A. hydrophila gidA gene, Apr Nmr
|
This study |
pGlow-Upact
|
pGlow carrying upstream region of A. hydrophila act gene, Apr Nmr
|
This study |
pKD46 |
λ Red recombinase plasmid, Apr
|
Datsenko and Wanner(2000) |
pKD3 |
cat cassette template with FRT sites, Apr Cmr
|
Datsenko and Wanner(2000) |
pCP20 |
FLP recombinase plasmid, Apr Cmr
|
Datsenko and Wanner(2000) |
pBAD/Thio-E |
Prokaryotic expression vector, Apr
|
Invitrogen |
pBAD-act
|
act gene of A. hydrophila cloned into pBAD/Thio-E, Apr
|
This study |