Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| A. hydrophila SSU-R strains | Rifampin resistance strain, Rifr | Laboratory |
| Parental | pBAD plasmid, Rifr Apr | Erova et al. (2006b) |
| Dam-overproducing | pBAD-dam plasmid, Rifr Apr | Erova et al. (2006b) |
| E. coli K12 strains | ||
| TOP10 | F− mcrA Δ mrr-hsdRMS-mcrBC Φ80lacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| GM28 | F− sup-85 (Am) | Marinus and Morris(1973) |
| GM33 | F− dam-3 sup-85 (Am) | Marinus and Morris(1973) |
| GM28 (pGlow-UpgidA) | GM28 with pGlow-UpgidA plasmid | This study |
| GM33 (pGlow-UpgidA) | GM33 with pGlow-UpgidA plasmid | This study |
| GM28 (pGlow-Upact) | GM28 with pGlow-Upact plasmid | This study |
| GM33 (pGlow-Upact) | GM33 with pGlow-Upact plasmid | This study |
| GM28 (pGlow-TOPO) | GM28 with pGlow-TOPO vector | This study |
| GM33 (pGlow-TOPO) | GM33 with pGlow-TOPO vector | This study |
| GM28 gidA mutant | In-frame gidA mutant of GM28 | This study |
| GM33 gidA mutant | In-frame gidA mutant of GM33 | This study |
| GM28 gidA mutant (pGlow-Upact) | GM28 gidA mutant carrying pGlow-Upact plasmid | This study |
| GM33 gidA mutant (pGlow-Upact) | GM33 gidA mutant carrying pGlow-Upact plasmid | This study |
| GM28 gidA mutant (pGlow-UpgidA) | GM28 gidA mutant carrying pGlow-UpgidA plasmid | This study |
| GM33 gidA mutant (pGlow-UpgidA) | GM33 gidA mutant carrying pGlow-UpgidA plasmid | This study |
| Plasmids | ||
| pGlow-TOPO | Promoterless vector, Apr Nmr | Invitrogen |
| pGlow-UpgidA | pGlow carrying upstream region of A. hydrophila gidA gene, Apr Nmr | This study |
| pGlow-Upact | pGlow carrying upstream region of A. hydrophila act gene, Apr Nmr | This study |
| pKD46 | λ Red recombinase plasmid, Apr | Datsenko and Wanner(2000) |
| pKD3 | cat cassette template with FRT sites, Apr Cmr | Datsenko and Wanner(2000) |
| pCP20 | FLP recombinase plasmid, Apr Cmr | Datsenko and Wanner(2000) |
| pBAD/Thio-E | Prokaryotic expression vector, Apr | Invitrogen |
| pBAD-act | act gene of A. hydrophila cloned into pBAD/Thio-E, Apr | This study |