Figure 2. Locomotion in two Drosophila ion channel mutants: para^ts1, and Hk¹.

(A) Four male flies were placed in an arena and tracked for 15 minutes. Shown are sample tracks from individual flies at four 30 s intervals. (B) The mean cumulative distance traveled for individuals from each of the three genotypes. Shaded regions represent S.E.M. Hk¹ and para^ts1 showed significantly altered patterns of locomotion with increased and decreased total distances traveled respectively when compared to CS (p < 0.01, Mann-Whitney U Test, n=40, 32, 24 flies for CS, Hk¹ and para^ts1 respectively) (C) The proportion of time individuals were moving, defined to be when their velocity was <3 mm/s. Error bars represent standard error of proportion (** p < 0.01, *** p < 0.001, Mann-Whitney U Test). (D) The cumulative distribution of fly velocities indicate the proportion of time a fly is moving (velocity > 3 mm/s), but is traveling slower than the corresponding velocity. The velocity profiles for CS, Hk¹, and para^ts1 were not significantly different, (Kolmogorov-Smirnov test, p > 0.05).