(A) Sample 30-s tracks of a male CS, olfD, and Gr32a fly approaching the virgin target flies (on right). (B) 15-min interactograms from the sample videos shown in (A). (C) Sample log-place map from videos in (A). (D) Bar graphs of the average amount of time male flies interact with targets during a 15-min video. Male olfD mutants spent far less time interacting than either CS or Gr32a males (Student’s T test, *p < 0.05, n= 15, 30, and 15 flies respectively for CS, olfD, and Gr32a). (E) The preference index of the mutants studied. Values greater than 0 indicate preference towards virgin targets, while those less than 0 indicate a preference towards mated targets. CS and Gr32a males showed a significant preference towards virgin targets (Student’s T-Test, *p < 0.05).