Figure 4.
Expression of IL12B and IL23A mRNA were increased in lesional psoriatic skin, whereas IL12A expression was decreased (A) (n = 7–10). Measurements of IL-12 (IL-12p70) and IL-12p40 from tissue lysates obtained from control (NN), uninvolved (PN) and lesional psoriatic skin (PP) (n = 9–13) revealed significantly increased protein levels of both IL-12p70 and IL-12p40 in lesional psoriatic skin (B). All mRNA values are expressed relative to the housekeeping gene RPLP0 as mean ± SEM. Protein values are expressed relative to total protein tissue lysate (1 mg/ml) as mean ± SEM. Statistical significance of two-tailed t-test indicated by one (P < 0.05) or two (P < 0.01) asterisks.