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Dithiothreitol (DTT) and reduced glutathione (GSH) improve respiratory growth of many yeast mutants. Strains showing a strong reductant-dependent growth stimulation in a systematic screen were grown overnight in galactose-containing medium and adjusted to optical density (OD) 1. Tenfold serial dilutions were spotted onto YPD (glucose) or YPG (glycerol) plates containing the indicated amounts of DTT and GSH. Plates were incubated for 2 (glucose) or 4 (glycerol) days at 25°C or 30°C.
