Table 1.
Growth Stimulation of Yeast Mutants on YPG in the Presence of 5 μM Reduced Glutathione or Dithiothreitol
| ORF | Gene | YPG 25°C | YPG 30°C | GSH 25°C | GSH 30°C | DTT 25°C | DTT 30°C | Function |
|---|---|---|---|---|---|---|---|---|
| Respiratory chain function and assembly | ||||||||
| YLR218C | COA4 | Poor | Poor | Strong | Strong | Strong | Strong | Twin cx9c protein involved in cytochrome c oxidase assembly or stability |
| YKL137W | CMC1 | None | None | Strong | Strong | Strong | Strong | Twin cx9c protein involved in cytochrome c oxidase assembly or stability |
| YML120C | NDI1 | Poor | Reduced | Minor | Minor | Minor | Minor | Internal NADH-ubi oxidoreductase |
| YDL107W | MSS2 | Reduced | Reduced | Strong | None | Strong | None | Cytochrome c oxidase assembly |
| YER058W | PET117 | Reduced | Reduced | Strong | None | Minor | None | Cytochrome c oxidase assembly |
| YOL096C | COQ3 | Poor | Reduced | None | Minor | None | Minor | Ubiquinone biosynthesis |
| Mitochondrial protein synthesis | ||||||||
| YDR025W | RPS11A | Reduced | Reduced | Strong | Strong | Strong | Strong | Mitochondrial ribosome SSU |
| YJL063C | MRPL8 | Poor | Reduced | Strong | Strong | Minor | Strong | Mitochondrial ribosome LSU |
| YMR097C | MTG1 | Poor | Reduced | Minor | Minor | Minor | Minor | GTPase required for biogenesis of mitochondrial ribosomes |
| YMR098C | ATP25 | None | Reduced | Minor | Minor | Minor | Minor | Required for Atp9 synthesis |
| YOR200W | Poor | Reduced | Minor | Minor | Minor | Minor | Overlaps with MRM1, mitochondrial ribose rRNA methyltransferase | |
| YCR024C | SLM5 | Reduced | Reduced | Minor | Strong | None | Minor | Mitochondrial asparaginyl-tRNA synthetase |
| YJR113C | RSM7 | Reduced | Reduced | Strong | Minor | None | None | Mitochondrial ribosome SSU |
| Other metabolic enzymes | ||||||||
| YJL121C | RPE1 | Reduced | Reduced | Strong | Minor | Strong | Minor | D-ribulose-5-phosphate 3-epimerase |
| YIL155C | GUT2 | Reduced | Reduced | Strong | Minor | Strong | None | Mitochondrial glycerol-3-phosphate dehydrogenase |
| YGR155W | CYS4 | Poor | Poor | Strong | Strong | Minor | None | Cystathionine beta-synthase |
| YJL120W | Reduced | Reduced | Minor | None | Strong | None | Partially overlaps the verified gene YJL121C/RPE1 | |
| YDL114W | Reduced | Reduced | Strong | None | Strong | None | Similarity to acyl-carrier-protein reductases | |
| YHR067W | HTD2 | Poor | Poor | Strong | None | Minor | Mitochondrial 3-hydroxyacyl-thioester dehydratase | |
| YDR148C | KGD2 | None | Poor | Minor | None | Minor | None | Mitochondrial alpha-ketoglutarate dehydrogenase complex |
| YIL125W | KGD1 | None | Poor | Minor | None | Minor | None | Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex |
| Nuclear gene expression | ||||||||
| YDR010C | Reduced | Reduced | Strong | Strong | Strong | Strong | Dubious, overlaps with Gal3 transcription factor | |
| YJL176C | SWI3 | Poor | Reduced | Strong | Minor | Minor | None | SWI remodeling complex |
| YCR020W-B | HTL1 | Reduced | Reduced | Minor | Minor | Minor | Minor | Component of the RSC chromatin remodeling complex |
| YOR380W | RDR1 | Reduced | Reduced | None | Minor | None | Strong | Transcriptional repressor nucleus |
| YOL051W | GAL11 | Reduced | Reduced | Strong | Minor | None | None | Transcription nucleus |
| YPL254W | HFI1 | Reduced | Reduced | Strong | None | Minor | None | SAGA complex nucleus |
| YGL066W | SGF73 | Reduced | Reduced | Minor | Minor | Minor | None | SAGA complex nucleus |
| YOL004W | SIN3 | Reduced | Reduced | None | Minor | None | Minor | Histone deacetylase |
| YGL070C | RPB9 | None | None | None | Minor | None | Minor | RNA polymerase II subunit B12.6 nucleus |
| Vacuolar transporters | ||||||||
| YDL128w | VCX1 | Reduced | Reduced | Strong | None | Strong | None | Vacuolar membrane antiporter with Ca2+/H+and K+/H+exchange activity |
| YOR332W | VMA4 | Reduced | Reduced | None | Strong | None | Minor | V-ATPase |
| YKL080W | VMA5 | Reduced | Reduced | Strong | None | None | Minor | V-ATPase |
| YPR036W | VMA13 | None | Reduced | None | Minor | None | Strong | V-ATPase |
| Other functions | ||||||||
| YBR283C | SSH1 | Reduced | Reduced | Strong | Strong | Strong | Strong | Sec61 homolog |
| YHR030C | SLT2 | Reduced | Reduced | Strong | Strong | Strong | Strong | MAP kinase, regulates autophagy |
| YIL153W | RRD1 | Reduced | Reduced | Strong | Minor | Strong | None | Peptidyl-prolyl cis/trans-isomerase |
| YKL040C | NFU1 | Poor | Reduced | Minor | Strong | None | None | Iron-sulfur cluster formation in mitochondria |
| YBL082C | ALG3 | Reduced | Reduced | Strong | None | Minor | None | Dolichol-P-Man dependent alpha(1–3) mannosyltransferase |
| YJL101C | GSH1 | Poor | Poor | Strong | None | Minor | None | Glutathion-synthase |
| Function unknown | ||||||||
| YCL001W-A | Reduced | Reduced | Strong | Strong | Strong | Strong | Function unknown | |
| YHR039C | MSC7 | Reduced | Reduced | Strong | None | Minor | Strong | Function unknown, ER-associated |
| YJR120W | Reduced | Reduced | Minor | Strong | None | Strong | Protein of unknown function; transporters Aus1p and Pdr11p | |
| YDR458C | HEH2 | Reduced | Reduced | None | Strong | Minor | None | Inner nuclear membrane (INM) protein, function unknown |
GSH, reduced glutathione.