Table 2.
Name | Nucleotide sequence | N-ter or C-ter protein sequence |
---|---|---|
TmPrxNtr for | 5′ CCCCCCATGGCTAGGGTGAAGCACTTTGAA 3′ | MARVKHFE |
TmPrxNtr rev | 5′ CCCCGGATCCCTAAAGGTTCCACCTGAC 3′ | VRWNL |
TmPrxNtr C40S for | 5′ GCAGGAACGAGCGGTAGCACGAGAGAAGCCGTC 3′ | |
TmPrxNtr C40S rev | 5′ GACGGCTTCTCTCGTGCTACCGCTCGTTCCTGC3′ | |
TmPrxNtr-Prx rev | 5′ CCCCGGATCCTTAGTCTTCTTCTATCAGTCG 3′ | RLIEED |
TmPrxNtr-Ntr for | 5′ CCCCCCATGGCTTTGAAAAAAGACAGAGTACCG 3′ | MALKKDRVP |
TmPrxNtr-Ntr for2 | 5′ CCCCCCATGGCTCTGAACAAGCACATAGAG 3′ | MALNKHIE |
TmTR for | 5′ CCCCCCATGGTTTTCTTTGATACTGGATCC 3′ | MVFFDTGS |
TmTR rev | 5′ CCCCCCATGGTCAGAAGTAATGTTTTGCGGC 3′ | AAKHYF |
TmGrx1 for | 5′ CCCCCCATGGCTAAAAAGGTGGAGATACTA 3′ | MAKKVEIL |
TmGrx1 rev | 5′ CCCCGGATCCTTAAGCCTGCTGAAGGACCTT 3′ | KVLQQA |
TmGrx2 for | 5′ CCCCCCATGGCTCAGCACCTGAAAATCAAA 3′ | MAQHLKIK |
TmGrx2 rev | 5′ CCCCGGATCCTTACGATATTCCAAGGAG 3′ | LLGIS |
TmGrx3 for | 5′ CCCCCCATGGGTATTTTGTCGGAC 3′ | MGILSD |
TmGrx3 rev | 5′ CCCCGGATCCTCACCCCTTTGCGAGTCT 3′ | RLAKG |
TmPrxBCP for | 5′ CCCCCCATGGCTTTGAAATCAGGCGATAAG 3′ | MALKSGDK |
TmPrxBCP rev | 5′ CCCCGGATCCTTATTTTGAAAGACGCTCGAG 3′ | LERLSK |
TmPrxBCP C50S for | 5′ TGTGAGAAAGAACTCTCCACGTTCAGGGATTCT 3′ | |
TmPrxBCP C50S rev | 5′ AGAATCCCTGAACGTGGAGAGTTCTTTCTCACA 3′ | |
TmPrx6 for | 5′ CCCCCCATGGCTGAAGGAAGAATTCCTCTC 3′ | MAEGRIPL |
TmPrx6 rev | 5′ CCCCGGATCCTTATACCTTTTTGTAACA 3′ | CYKKV |
The NcoI and BamHI restriction sites are underlined, and the mutagenic codons as well as the GCT codon that are added in some sequences to keep them in frame are in bold characters.