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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Cell Biochem. 2010 Dec 1;111(5):1280–1290. doi: 10.1002/jcb.22852

Figure 3. p53 colocalized with PML in irradiated U2OS cells.

Figure 3

U2OS cells on glass coverslips were either untreated (NT, A) or treated with irradiation (IR, 10Gy, B,C). Cells were fixed with 4% formaldehyde at indicated time points. P53, p21 antibodies were detected using an Alexa-488 conjugated goat anti rabbit IgG. PML antibody were detected using a rhodamine-x conjugated goat anti mouse IgG. Cell nuclei were counterstained with DAPI (blue). Images were captured at 100× magnification. Representative images were shown. Representative foci with p53/PML colocalization were pointed with white arrows. Representative confocal images were shown in C.