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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Cell Biochem. 2010 Dec 1;111(5):1280–1290. doi: 10.1002/jcb.22852

Figure 6. p21 colocalized with PML in Nutlin treated U2OS cells.

Figure 6

U2OS cells on glass coverslips were continuously treated with Nutlin (5 μM). Cells were fixed with 4% formaldehyde at indicated time points. A) P53, p21 antibodies were detected using an Alexa-488 conjugated goat anti rabbit IgG. PML antibody were detected using a rhodamine-x conjugated goat anti mouse IgG. Representative foci with p21/PML colocalization were pointed with white arrows. B) Percentage of cells with p53/PML, p21/PML, p53/p21/PML colocalization was determined at indicated time points after treatment. Percentage represents Mean ± SEM of three independent experiments with 200 cells counted per experiment. C) Representative confocal images were shown.