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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Cell Biochem. 2010 Dec 1;111(5):1280–1290. doi: 10.1002/jcb.22852

Figure 7. p21 colocalized with γH2AX in Nutlin treated U2OS cells.

Figure 7

U2OS cells on glass coverslips were treated as described in Figure 4. A) Percentage of cells with >10 γH2AX foci was determined at indicated time points after treatment. Percentage represents Mean ± SEM of three independent experiments with 200 cells counted per experiment. B) p21, PML antibodies were detected using an Alexa-488 conjugated goat anti rabbit IgG. γH2AX antibody were detected using a rhodamine-x conjugated goat anti mouse IgG. Representative colocalization foci were pointed with white arrows.