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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Comp Neurol. 2010 Dec 1;518(23):4723–4739. doi: 10.1002/cne.22472

Figure 3.

Figure 3

Categorization of postembedding immunogold labeling (PEG) in relation to the postsynaptic density (PSD; gray fill) of asymmetric synapses. PEG particles positioned at the cleft, over the PSD or near the PSD (<60 nm from the postsynaptic membrane surface) were categorized as “postsynaptic.” PEG particles at positions removed from the postsynaptic membrane surface by more than 60 nm were all categorized as “nonsynaptic.” The “nonsynaptic” PEG particles included those residing in the spine neck, sometimes as much as a micrometer removed from the PSD. PEG particles residing along the plasma membrane or in the intracellular cytoplasm were included in the nonsynaptic category. The total number of PEG particles residing postsynaptically and nonsynaptically was categorized as “total spinous” for spine profiles.