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. 2013 Apr 1;8(4):e58797. doi: 10.1371/journal.pone.0058797

Figure 1. Glucokinase activity in soluble fractions of liver and hypothalamus.

Figure 1

Extracts were prepared from liver and hypothalamus of female mice of the indicated genotypes. GK activity was calculated by subtracting the glucose-phosphorylating activity at 0.3 mM glucose from the total activity measured at 30 mM glucose. The bars represent means ± SE. n = 8 IRS-2(−/−) (6–7 weeks old), n = 10 WT (6–7 weeks old), n = 5 RIP-Irs-2/IRS-2(−/−) at ∼16 months of age, and n = 5 WT (at ∼16 months of age). GK activity in liver from wild type mice (6–7 weeks old) was considered as 1. *** p<0.001 (IRS-2(−/−) vs WT mice).