Skip to main content
. 2013 Apr 1;8(4):e60205. doi: 10.1371/journal.pone.0060205

Figure 5. Bisulphite sequencing analysis of LINE1, Lemd1, Nanog, Oct4 and H19-DMR in GSE-KD oocytes and zygotes.

Figure 5

(A) Bisulphite sequencing of CpG sites within LINE1 genomic regions in spermatozoa and in untreated and GSE-KD oocytes and zygotes at PN3–4. (B) Bisulphite sequencing of CpG sites within Lemd1 genomic regions in spermatozoa and in untreated and GSE-KD oocytes and zygotes at PN3–4. (C) Bisulphite sequencing of CpG sites within Nanog genomic regions in spermatozoa and in untreated and GSE-KD oocytes and zygotes at PN3–4. The methylation profile of the CpG sites in detail is indicated in Figure S8. (D, E, F) Bisulphite sequencing of CpG sites within the upstream distal enhancer (DE) (D), proximal enhancer (PE) (E) and promoter regions (F) of the Oct4 gene in spermatozoa and untreated and GSE-KD oocytes and zygotes at PN3–4. (G) Bisulphite sequencing of CpG sites within the H19-DMR in sperm and untreated and GSE-KD oocytes and zygotes at PN3–4.