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. 2013 Apr 1;8(4):e60823. doi: 10.1371/journal.pone.0060823

Figure 5. Releasing adherent leukemia cells enhances cell killing by chemotherapy.

Figure 5

(A) Representative flow plots showing that adherent leukemia cells that survived Ara-C treatment re-enter cell cycle upon release from activated ECs. Cell cycle status was determined based on BrdU uptake. (B) Addition of anti-E-selectin antibody to adherent KG-1 cells significantly increased the number of released cells compared to IgG treated controls. (C) Adherent KG-1 cells were treated with E-selectin blocking antibody or an irrelevant IgG control and exposed to 200 µM Ara-C. The total number of Annexin V positive released leukemia cells is increased in the presence of anti-E-selectin antibody following treatment. * p<0.05.