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cDNA analysis of PCR products resulting from the amplification of cDNA from subjects harboring the IVS25+1G>A mutation versus wild type controls, visualised after electrophoresis on a 2% ethidium-bromide stained agarose gel. Ladder; Lanes 2–3: No Template Control (NTC); Lanes 4–5: two wild-type subjects for the IVS25+1G>A mutation; Lanes 6–9 : subjects heterozygous for the IVS25+1G>A mutation.
