Table 1. Primers used for PCR amplification of the exons and exon-intron boundaries of the ACE gene (accession number AY436326).
Name | Sequence (5′->3′) | Position | Expected amplicon size (bp) |
ACE1-FOR | GGGGGTGTGTCGGGTT | 2296–2311 | 253 |
ACE1-REV | TCGGCGCTGGAGTTGTA | 2532–2548 | |
ACE2-FOR | CCTTTATGGCCTGCATCTA | 3151–3170 | 1285 |
ACE2-REV | GGGCAATGTGGGAACACT | 4417–4435 | |
ACE3-FOR | GTGAAGGCCGTTGAAGACT | 4986–5004 | 865 |
ACE3-REV | ACTTCCGTGGGACTCATG | 5832–5850 | |
ACE4-FOR | ACTCAGCGATGCATGAAGAA | 6301–6320 | 743 |
ACE4-REV | AGAACGTGCTCTTCCCATGT | 7026–7045 | |
ACE5-FOR | TGTCTTTCAGGTGCCAATC | 7658–7676 | 332 |
ACE5-REV | CCTCTCAGCCCTCCCATA | 7972–7989 | |
ACE6-FOR | TTCCCTCTCCTTTCATCTCA | 8279–8298 | 1060 |
ACE6-REV | CATCAGAGCAGCCAGACA | 9321–9338 | |
ACE7-FOR | GCCCATGGTACCCACTCT | 9584–9601 | 1131 |
ACE7-REV | GGGACAGCATCTCTGTGTGT | 10696–10715 | |
ACE8-FOR | AGCCCTCAGCTCCCACTT | 11753–11770 | 632 |
ACE8-REV | CTCACTCAGCCTGCACAT | 12366–12384 | |
ACE9-FOR | CAAGCAGAGGTGAGCTAAGG | 13878–13897 | 585 |
ACE9-REV | CACTGTGGTCCGTCTTTACC | 14442–14461 | |
ACE10-FOR | GAGGGCATTGAGCCTAAGTA | 16171–16190 | 562 |
ACE10-REV | GACCCTTTGCTTGGTTCAT | 16715–16733 | |
ACE11-FOR | CACTGTCCTCTCCCAACAC | 18672–18690 | 1135 |
ACE11-REV | ACACAAAGCTGTGGGTACTG | 19787–19806 | |
ACE12-FOR | AAGGCCCTCAACCAACTC | 21626–21643 | 680 |
ACE12-REV | GTTAAGACCCAAGGCTGGAG | 22286–22305 | |
ACE13-FOR | CATGCTGTCTCCTTGCTT | 22391–22408 | 262 |
ACE13-REV | GTCACCTCAGGAGTGTCTCA | 22634–22653 |