Table 1. Primers used for PCR amplification of the exons and exon-intron boundaries of the ACE gene (accession number AY436326).

Name	Sequence (5′->3′)	Position	Expected amplicon size (bp)
ACE1-FOR	GGGGGTGTGTCGGGTT	2296–2311	253
ACE1-REV	TCGGCGCTGGAGTTGTA	2532–2548
ACE2-FOR	CCTTTATGGCCTGCATCTA	3151–3170	1285
ACE2-REV	GGGCAATGTGGGAACACT	4417–4435
ACE3-FOR	GTGAAGGCCGTTGAAGACT	4986–5004	865
ACE3-REV	ACTTCCGTGGGACTCATG	5832–5850
ACE4-FOR	ACTCAGCGATGCATGAAGAA	6301–6320	743
ACE4-REV	AGAACGTGCTCTTCCCATGT	7026–7045
ACE5-FOR	TGTCTTTCAGGTGCCAATC	7658–7676	332
ACE5-REV	CCTCTCAGCCCTCCCATA	7972–7989
ACE6-FOR	TTCCCTCTCCTTTCATCTCA	8279–8298	1060
ACE6-REV	CATCAGAGCAGCCAGACA	9321–9338
ACE7-FOR	GCCCATGGTACCCACTCT	9584–9601	1131
ACE7-REV	GGGACAGCATCTCTGTGTGT	10696–10715
ACE8-FOR	AGCCCTCAGCTCCCACTT	11753–11770	632
ACE8-REV	CTCACTCAGCCTGCACAT	12366–12384
ACE9-FOR	CAAGCAGAGGTGAGCTAAGG	13878–13897	585
ACE9-REV	CACTGTGGTCCGTCTTTACC	14442–14461
ACE10-FOR	GAGGGCATTGAGCCTAAGTA	16171–16190	562
ACE10-REV	GACCCTTTGCTTGGTTCAT	16715–16733
ACE11-FOR	CACTGTCCTCTCCCAACAC	18672–18690	1135
ACE11-REV	ACACAAAGCTGTGGGTACTG	19787–19806
ACE12-FOR	AAGGCCCTCAACCAACTC	21626–21643	680
ACE12-REV	GTTAAGACCCAAGGCTGGAG	22286–22305
ACE13-FOR	CATGCTGTCTCCTTGCTT	22391–22408	262
ACE13-REV	GTCACCTCAGGAGTGTCTCA	22634–22653