Table 2. Number of sequences assembled by NGS of vegetal DNA extracted from tapir faeces (N = 39, 79×60 mg of faecal product), using plant universal primers targeting the P6 loop region of the trnL (UAA) intron and primers specific for the Sapotaceae family, targeting the first internal transcribed spacer (ITS1) of nuclear ribosomal DNA.
Primer pair | g / h (1) | ITS1-F / ITS1Sap-R (2) |
Number of properly assembled sequences | 3246036 | 77884 |
Number of unique sequences | 70588 | 15183 |
Number of unique sequences longer than 10 (1) or 40 (2) bp | 70552 | 15162 |
Number of unique sequences longer than 10 (1) or 40 (2) bp, and with occurrence higher or equal to 11 | 5488 | 257 |
Number of unique sequences after cleaning of the artefacts | 117 | 12 |
Corresponding total number of sequences analysed | 2598249 | 15614 |