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. 2013 Apr 1;8(4):e60799. doi: 10.1371/journal.pone.0060799

Table 2. Number of sequences assembled by NGS of vegetal DNA extracted from tapir faeces (N = 39, 79×60 mg of faecal product), using plant universal primers targeting the P6 loop region of the trnL (UAA) intron and primers specific for the Sapotaceae family, targeting the first internal transcribed spacer (ITS1) of nuclear ribosomal DNA.

Primer pair g / h (1) ITS1-F / ITS1Sap-R (2)
Number of properly assembled sequences 3246036 77884
Number of unique sequences 70588 15183
Number of unique sequences longer than 10 (1) or 40 (2) bp 70552 15162
Number of unique sequences longer than 10 (1) or 40 (2) bp, and with occurrence higher or equal to 11 5488 257
Number of unique sequences after cleaning of the artefacts 117 12
Corresponding total number of sequences analysed 2598249 15614