Figure 3. ¹³C enrichment in expired CO₂ of embryos incubated at two different eggshell temperatures (study III).

¹³C enrichment in expired CO₂ after injecting a single bolus of [U-¹³C]glucose (1.0 mg in 250 µL sterile water) at day 17.5 of incubation in the chorio-allantoic fluid of embryos incubated at a normal (37.8°C) or high (38.9°C) eggshell temperature (EST) from day 10.5 of incubation onward. From day 17.6 until 17.8 of incubation, ¹³C enrichment in expired CO₂ was higher for the high EST compared to the normal EST treatment (P<0.05).