Figure 4. ¹³C enrichment in plasma glucose of embryos incubated at two different eggshell temperatures (study III).

¹³C enrichment in plasma glucose after injecting a single bolus of [U-¹³C]glucose (1.0 mg in 250 µL sterile water) at day 17.5 of incubation in the chorio-allantoic fluid of embryos incubated at a normal (37.8°C) or high (38.9°C) eggshell temperature (EST) from day 10.5 of incubation onward. At day 17.5, 17.8, 18.2, and 18.9 of incubation, no chickens were hatched yet. At day 19.7 of incubation, 22% of the chickens of the high EST treatment and 10% of the chickens of the normal EST treatment were hatched. At day 20.4 of incubation, 80% of the chickens of the high EST treatment were hatched and all chickens of the normal EST treatment were hatched. At day 20.9 and 21.6 of incubation, all chickens had been hatched.