Figure 1. The glycosylation induced under glucose deprivation was not regulated by OGT and O-GlcNAcase.
A, An immunoblot showing CTD110.6 antibody and anti-CD98HC antibody reaction over the time course during incubation of T24 cells in glucose-deprived medium (0 mM glucose). B, The effects of tunicamycin treatment on the expression of proteins that reacted with CTD110.6 and anti-CD98HC antibodies under glucose deprivation. An anti-α-tubulin antibody was used as an internal control. Note that N-GlcNAc2-modified glycoproteins are undetectable after treatment with tunicamycin in medium containing no glucose (0 mM glucose medium). Arrow shows N-GlcNAc2-modified forms CD98HC.