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. 2013 Apr 1;8(4):e60397. doi: 10.1371/journal.pone.0060397

Figure 7. Quantitative RT-PCR of five mitotic kinase genes, under siRNA targeting for ATF3, ATF4 or FOXO1.

Figure 7

Quantitative RT-PCR was performed on T24 cells in which siRNA targeted ATF3, ATF4 or FOXO1. The cells were either incubated in 25 mM or 0 mM glucose medium for 1 day. Gene expression was normalized using GAPDH transcripts. Error bars represent standard errors from three independent experiments. * and **: signify p<0.05 and p<0.01, respectively, against 25 mM glucose with the control-scrambled RNA duplex (siCont) treatment. # and ##: signify p<0.05 and p<0.01, respectively, against 0 mM glucose samples with siCont treatment. Note that the expression of five mitotic kinase genes under glucose deprivation was suppressed. FOXO1 knockdown inhibited the expression of the five mitotic kinase genes when the cells were incubated in 25 mM glucose medium. Otherwise, knockdown of ATF3 or ATF4 allowed the expression of the five mitotic kinase genes to recover when the cells were incubated in medium lacking glucose (0 mM glucose condition).