Figure 1. Flg22 pretreatment suppressed FB1-triggered cell death.

Twenty-four hours after pretreatment with water (mock) or 1 µM flg22, 1% methanol (mock) or 50 µM FB1 were infiltrated into the pretreated leaves of four-week old plants. Three days after FB1 treatment, the leaves were subjected to macroscopic examination (A) or trypan blue staining (B) to visualizeFB1-triggered cell death. Scale bar  =  1 cm. (C) Quantification of FB1-triggered cell death by electrolyte leakage. Leaf disks (6 mm diameter) were prepared 48 hours after FB1 treatment. Four leaf disks, from two leaves, were floated on in wells of 12-well plate each containing 2 ml of purified water. Bars represent means and standard errorsfor biological replicates, calculated using a mixed linear model: four to six biological replicates were performed for each of two independent experiments. The vertical axis shows the log₁₀-transformed conductivity values. Asterisks indicate significant differences from the mock-pretreatment (P<0.001, two-tailed t-tests).