Figure 1. Cellular handling of labeled galactose.

Myotubes were grown in DMEM-media with 5.5 mM glucose (A) or 5.5 mM galactose (B). For cellular uptake (n = 4) (C) and oxidation (CO₂-trapping) (n = 4) (D) the cells were exposed to [1-¹⁴C]galactose (1 µCi/ml, 200 µM) for 4 h before harvesting. For lipogenesis (n = 6) (E) the cells were exposed to [U-¹⁴C]glucose (1 µCi/ml, 200 µM) or [1-¹⁴C]galactose (1 µCi/ml, 200 µM) for 24 h before harvesting as described in Materials and Methods. Values represent nmol/mg cell protein given as means ± SEM. *P<0.05 vs. glucose pretreatment.