Figure 2. Effect of galactose treatment on oleic acid metabolism.

Myotubes were either grown in DMEM-media with 5.5 mM glucose or 5.5 mM galactose during the whole seeding period, or with 5.5 mM glucose during proliferation and 5.5 mM galactose during differentiation (Galactose_d). Thereafter, the cells were exposed to [1-¹⁴C]oleic acid (1 µCi/ml, 100 µM) for 4 h as described in Materials and Methods. The figures show cellular uptake (n = 6) (A), oxidation (n = 6) (B), % oxidized (CO₂/CA+CO₂) (n = 6) (C) and suppressibility (n = 3) (D) of [1-¹⁴C]oleic acid. Suppressibility, defined as the ability of the cells to decrease oleic acid oxidation by acutely added glucose, was calculated as: [(1-(oxidation of oleic acid at 5 mM glucose/oxidation of oleic acid at no glucose added))×100%]. Values represent means ± SEM. *P<0.05 vs. glucose.