Figure 4. TAC inhibits NF-κB DNA-binding capacity in human T cells.

MACS isolated CD3+ T cells (3.10⁶) from three healthy volunteers were stimulated in the presence of TAC 0, 10, 50 ng/mL or sotrastaurin 500 nM. A) The amount of nuclear protein extracted from the cells was comparable in the samples and at the different tested conditions. B) The DNA binding capacity of NF-κB in the nuclear fractions was inhibited by both TAC and the positive control sotrastaurin. Each bar represents the anti-CD3/CD28-stimulated NF-κB DNA binding capacity minus that of the unstimulated sample (mean ± SEM of 3 independent experiments).