Figure 4. Phylogeny of vertebrate GCKR protein sequences.

Phylogeny of vertebrate GCKR protein sequences generated from intact and near-full length GCKR coding sequences (see Figure S1). Only select mammals were included in the analysis. The bootstrapped (1000 replications) neighbor-joining distance tree was generated using JTT protein distances. The Lamprey (jawless fish) sequence was used as the outgroup. Similar trees were generated when different protein distance measures were used or if other tree building methods, such as parsimony or maximum likelihood, were used.