Figure 2.

CLEM of pSEO2.HDEL:GFP. A, TEM image of an ultrathin section of petiole from a plant expressing pSEO2.HDEL:GFP. The section was poststained with uranyl acetate and lead citrate. Scale = 5 μm. B, A semithin section acquired immediately after the TEM section, imaged with the confocal microscope, showing the same field of view. Note that small vacuoles in the cytoplasm can be seen in both the TEM and confocal images (stars in A and B). Scale = 5 μm. C, Overlay image of A and B showing alignment of sieve elements in the confocal and TEM images. D, A Semithin section of the phloem imaged in the confocal microscope shows conspicuous SER stacks (arrow). Scale = 10 μm. E, The TEM image of the same field of view. The same SER stack arrowed in D is apparent in E (arrow). Scale = 5 μm. F, An enlarged image of the SER stack arrowed in E. Scale = 1 μm.