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Coelution of PEPC activity with p110 and p107 during Superdex 200 FPLC of PEPC from stage I and II proteoid roots. Aliquots (1 μL) from various fractions along with purified PEPC (50 ng) from germinating COS (“Castor PEPC”) were subjected to immunoblotting with anti-PEPC. Vo denotes the void volume.
