Table I. Overview of Golgi N-glycan processing enzyme constructs used for 2P-FRET-FLIM.

For a full description of the plasmid constructs, see “Materials and Methods” and Supplemental Table S2. FL, Full length.

Abbreviation	Full Name	Tag	Domains
MNS1	Arabidopsis Golgi α-mannosidase I	GFP, mRFP	CTS, FL
GnTI	N. tabacum β1,2-N-acetylglucosaminyltransferase I	GFP, mRFP	CTS, FL
GMII	Arabidopsis Golgi α-mannosidase II	GFP, mRFP	CTS, FL
XylT	Arabidopsis β1,2-xylosyltransferase	GFP, mRFP	CTS
GALT1	Arabidopsis β1,3-galactosyltransferase1	GFP	CTS
FUT13	Arabidopsis α1,4-fucosyltransferase	mRFP	FL
ST	R. norvegicus α2,6-sialyltransferase	GFP, mRFP	CTS