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. 2013 Feb 20;161(4):1755–1768. doi: 10.1104/pp.112.208942

Figure 2.

Figure 2.

AtMMS21 is essential for QC organization, identity, and function. A and B, Longitudinal view of the stem cell niche of wild-type (WT) and mms21-1 roots at 5 DAG stained with PI showing cells of the QC (arrowhead) and CSCs (arrow). C and D, EdU incorporation assays in wild-type and mms21-1 root meristems. The QC cells are marked with green fluorescence in pWOX5:GFP, and EdU-positive nuclei are marked by red fluorescence. Wild-type QC cells do not have red fluorescent nuclei, indicating that the QC is not mitotically active; by contrast, red fluorescent EdU-positive nuclei in the mms21-1 QC cells indicate a state of active division (arrow). E to F, Expression pattern of pWOX5:GFP in the wild type and mms21-1 at 5 DAG. H to J, Double staining of the QC25:GUS marker and starch granules (purple) in 5-DAG wild type and mms21-1 seedlings. The arrowhead and arrow in the wild type indicate QC and columella initials, respectively. Arrows in mms21-1 indicate cells in the region of the QC and CSCs. Bars = 20 μm (A–G) and 50 μm (H–J).