Figure 7.

Spatial analysis of LepaDOG1 protein expression within the L. papillosum infructescence. A, Type I (left) and type II (right) L. papillosum infructescences, their experimental dissection into FO (each containing eight fruits and thereby 16 seeds), and their FO-specific germination time courses. Note that nongerminating seeds from top FOs are immature, while nongerminating seeds from bottom FOs are dormant. B, FO-specific immunoblot analysis of type I (left) and type II (right) infructescences shown in A displaying the protein expression patterns for LepaDOG1, oleosin (expressed during early maturation, indicative for seed filling), EM6, and dehydrin (expressed during late maturation, indicative for seed maturation and desiccation tolerance) by using corresponding antibodies (α-DOG1², α-oleosin, α-EM6, α-dehydrin). A Coomassie blue-stained gel showing the FO protein extract banding patterns is seen at bottom left. kD indicates the protein molecular mass marker ladder. [See online article for color version of this figure.]