Figure 2.

MeJA alters the evolution of the leaf area by affecting cell size and cell number differentially and in a COI1-dependent manner. Kinematic analysis of leaf growth of Col gl1, coi1-16B, and aos throughout their development is shown. Plants were grown in vitro in the absence (−) or presence (+) of 50 µm MeJA. Leaves 1 and 2 were harvested at 9, 13, and 19 DAS to measure the leaf area (A), the epidermal cell area (C), and the number of epidermal cells per leaf (E). MeJA inhibition (%) has been calculated for the leaf area (B), the epidermal cell area (D), and the number of epidermal cells per leaf (F). Values denote averages ± se of at least three biological replicates (n = 4–21, where n is the number of plants analyzed per biological replicate). In A, C, and E, continuous and dashed lines indicate untreated (−) and MeJA-treated (+) samples, respectively. C and E use a log₁₀ scale.