Figure 4.

MeJA alters cell cycle progression during leaf and cotyledon development. Cell cycle analysis of flow cytometry data was performed on the cotyledons at 10, 12, 14, 18, and 21 DAS in N. benthamiana (A) or on the first true pair of leaves (1+2) at 8, 10, and 12 DAS of Col gl1 and coi1-16B Arabidopsis plantlets (B). Seedlings were vertically grown in vitro on standard medium for 10 (A) or 8 (B) DAS and transferred to medium containing (+) or not containing (−) 50 μm MeJA. Seedlings growing on MeJA-containing medium were retransferred to MeJA-free medium (+/−) at 14 (A) or 10 (B) DAS. The values represent average frequencies of the observed cell cycle phases (G1, S, or G2/M) of at least five individual seedlings on independent biological replicates. Error bars indicate se.