Figure 6.

MeJA triggers a reduction of nucleus size in Arabidopsis root cells. Quantification of nuclear surface area visualized by H2B-YFP fusion proteins in 6-d-old roots untreated and treated with 50 µm MeJA was performed according to Ishida et al. (2010). A, Average nuclear area, defined as total pixel intensities of H2B-YFP signals per individual nucleus, was quantified within cells of the same epidermal layers in the meristem and the elongation zone of untreated and MeJA-treated roots. B, Nuclear area plotted against the distance from the QC. The dotted line indicates the average size at the time of the first endocycles. Representative results are shown for 6-d-old untreated (triangles) and MeJA-treated (diamonds) wild-type roots. Data were obtained from at least three biological replicates (n = 18–47).