Figure 3.

rcd1 does not enhance disease resistance at 22°C or 28°C in Arabidopsis. A, Growth of Pst DC3000 in Col-0, rcd1, snc1, and rcd1 snc1 at 22°C and 28°C. Shown is the growth of bacterial strains at 0 and 3 d post inoculation. Each genotype at each temperature had three biological repeats in each experiment. Values represent means ± sd (n = 3). The letter a indicates a significant difference from the wild-type Col-0 but the same between the two genotypes (P < 0.05 according to Student’s t test). Similar results were obtained in three independent experiments, although snc1 and rcd1 snc1, while behaving the same, did not always show significant differences from Col-0 at 28°C at 3 d post inoculation. cfu, Colony-forming units. B, Expression of EDS1 and PR1 is not altered by the rcd1 mutation in snc1 at either 22°C or 28°C assayed by RNA blots. Ribosomal RNA was used as a loading control. C, Quantification of SA levels in Col-0, snc1, rcd1, and rcd1 snc1. Plants were grown on soil for 3 weeks at 22°C and 28°C. Data are means ± sd (n = 3) from three biological repeats. Asterisks indicate significant differences from the wild-type Col-0 (P < 0.05). FWT, Fresh weight. D, SA and PAD4 are not required for the dwarf phenotype of rcd1. Shown are the wild-type Col-0, rcd1, snc1, rcd1 snc1, snc1 NahG, rcd1 NahG, rcd1 snc1 NahG, snc1 pad4, rcd1 pad4, and rcd1 snc1 pad4 plants grown at 22°C and 28°C. [See online article for color version of this figure.]