Figure 1.

Elevated AtPep1-triggered ROS production after pretreatment with flg22. A, Leaf discs were pretreated with indicated flg22 concentrations or without any peptide (control) for 16 h and then treated with 1 µm AtPep1 or without any peptide (control). Graphs represent mean values of ROS production in at least eight replicates. B and E, Leaf discs of Col-0 plants (B) and fls2 and efr mutants (E) were pretreated with the indicated elicitors (1 µm) or without any peptide (control) for 16 h and then treated with 1 µm of the indicated elicitor or without any peptide (control). C, Same as in B, but the pretreatment was performed for the indicated period of time. D, “Standard” was performed as in B, but in “washed,” leaf discs were washed before the final treatment. Columns represent averages of the peak values of ROS production of at least six biological replicates. Error bars show se of the mean. Asterisks represent Student’s t test results (*P < 0.05, **P < 0.01, ***P < 0.001). RLU, Relative light units; ns, not significant.