Figure 3.

Lack of MPK3 or MPK6 does not impair flg22-mediated elevation of AtPep1-triggered ROS production. A, Leaf discs of Col-0, mpk3, and mpk6 mutant plants were pretreated with either 1 µm flg22 or without any peptide (control) for 16 h and then treated with either 1 µm AtPep1, 1 µm flg22, or without any peptide (control) as indicated. Columns represent averages of the peak values of ROS production of 12 biological replicates. Error bars show se of the mean. Asterisks represent Student’s t test results (*P < 0.05). RLU, Relative light units.