Figure 3.

Ser-51 plays a critical role in the phosphorylation of HopQ1. Recombinant HopQ1 variants with a C-terminal 6xHis epitope were incubated with leaf protein extracts from N. benthamiana in buffer containing [γ-³²P]ATP. Samples were resolved by SDS-PAGE and analyzed by autoradiography. Only residual phosphorylation was detected for HopQ1 mutated at position 51. As a loading control, the Coomassie Brilliant Blue (CBB)-stained gel is shown in the bottom panel. This experiment was performed three times with similar results.