Table I. LC-MS/MS data showing 14-3-3 family isoforms that copurify with HopQ1-Strep II transiently expressed in N. benthamiana leaves.
| 14-3-3 Isoform | Sequence Coverage in Separate Experimentsa |
|||
|---|---|---|---|---|
| a | 28 | 14 | 60 | 43 |
| b | 40 | 29 | 0b | 0 |
| c | 28 | 16 | 50 | 50 |
| d | 30 | 0 | 0 | 0 |
| e | 28 | 54 | 54 | 0 |
| f | 27 | 27 | 0 | 0 |
| g | 30 | 23 | 0 | 0 |
| h | 47 | 0 | 0 | 0 |
| i | 30 | 31 | 0 | 0 |
a
Protein coverage parameters calculated by Mascot software are shown for each experiment. Protein coverage parameters for HopQ1 in these experiments were 61, 36, 23, and 65, respectively. bProtein coverage 0 refers to the 14-3-3 isoform that has not been identified by LC-MS/MS analyses in this experiment.